全文获取类型
收费全文 | 5658篇 |
免费 | 721篇 |
国内免费 | 3篇 |
出版年
2021年 | 84篇 |
2020年 | 41篇 |
2019年 | 51篇 |
2018年 | 61篇 |
2017年 | 53篇 |
2016年 | 98篇 |
2015年 | 170篇 |
2014年 | 187篇 |
2013年 | 231篇 |
2012年 | 279篇 |
2011年 | 297篇 |
2010年 | 154篇 |
2009年 | 136篇 |
2008年 | 266篇 |
2007年 | 255篇 |
2006年 | 243篇 |
2005年 | 243篇 |
2004年 | 211篇 |
2003年 | 197篇 |
2002年 | 187篇 |
2001年 | 203篇 |
2000年 | 212篇 |
1999年 | 157篇 |
1998年 | 79篇 |
1997年 | 77篇 |
1996年 | 63篇 |
1995年 | 61篇 |
1994年 | 76篇 |
1993年 | 78篇 |
1992年 | 147篇 |
1991年 | 134篇 |
1990年 | 129篇 |
1989年 | 113篇 |
1988年 | 105篇 |
1987年 | 121篇 |
1986年 | 98篇 |
1985年 | 102篇 |
1984年 | 96篇 |
1983年 | 71篇 |
1982年 | 63篇 |
1981年 | 66篇 |
1980年 | 48篇 |
1979年 | 67篇 |
1978年 | 51篇 |
1977年 | 43篇 |
1976年 | 46篇 |
1975年 | 39篇 |
1974年 | 42篇 |
1973年 | 58篇 |
1970年 | 34篇 |
排序方式: 共有6382条查询结果,搜索用时 125 毫秒
61.
Direct demonstration of actin filament annealing in vitro 总被引:6,自引:5,他引:1
Direct electron microscopic examination confirms that short actin filaments rapidly anneal end-to-end in vitro, leading over time to an increase in filament length at steady state. During annealing of mixtures of native unlabeled filaments and glutaraldehyde-fixed filaments labeled with myosin subfragment-1, the structural polarity within heteropolymers is conserved absolutely. Annealing does not appear to require either ATP hydrolysis or the presence of exogenous actin monomers, suggesting that joining occurs through the direct association of filament ends. During recovery from sonication the initial rate of annealing is consistent with a second-order reaction involving the collision of two filament ends with an apparent annealing rate constant of 10(7) M-1s-1. This rapid phase lasts less than 10 s and is followed by a slow phase lasting minutes to hours. Annealing is calculated to contribute minimally to filament elongation during the initial stages of self-assembly. However, the rapid rate of annealing of sonicated fixed filaments observed in vitro suggests that it may be an efficient mechanism for repairing breaks in filaments and that annealing together with polymer-severing mechanisms may contribute significantly to the dynamics and function of actin filaments in vivo. 相似文献
62.
An objective structured interview is an integral part of the process of selecting and admitting applicants to study medicine at this university. During the nine years (to the end of 1986) that the interview has been used 1600 candidates were interviewed out of roughly 13 000 applicants, and from these, 584 students were admitted to the course. Analysis of the interview data was carried out based on two aspects of student progress: graduation with honours and failure to complete the course of study.The interview as a whole, and especially some of the subscales, appears to identify students who may fail to complete the course: it may also help to predict which students are likely to graduate with honours. 相似文献
63.
64.
65.
66.
67.
68.
M E Murphy R Kolvenbach M Aleksis R Hansen H Sies 《Free radical biology & medicine》1992,13(2):95-100
To assess whether oxidative stress contributes to the ischemia/reperfusion injury of aortic surgery, the contents of alpha-tocopherol, alpha-tocopheryl quinone, ascorbate, lipid-derived malondialdehyde, protein thiols, cholesterol, and lactate were analyzed in plasma samples from 24 patients subjected to aortic crossclamping. alpha-Tocopherol, ascorbate, and protein thiols decreased during ischemia, whereas alpha-tocopheryl quinone increased in all but two cases, doubling on average in proportion to alpha-tocopherol. Upon reperfusion alpha-tocopherol, ascorbate, and protein thiols remained low, whereas alpha-tocopheryl quinone returned to the preischemic level. Lipid-derived malondialdehyde (a measure of lipid hydroperoxides) increased significantly only during reperfusion. The results suggest that oxidative stress occurs simultaneously with ischemia/reperfusion during aortic surgery, and that measurement of the tocopheryl quinone/tocopherol ratio may shed new light on the underlying pathological events. 相似文献
69.
J S Gray T M Murphy K A Waldrup G G Wagner D A Blewett R Harrington 《Journal of wildlife diseases》1991,27(1):86-91
Babesia odocoilei from white-tailed deer (Odocoileus virginianus) in Texas (USA) and B. capreoli isolated from sika deer (Cervus nippon) in Ireland were compared morphologically and antigenically. Babesia odocoilei and B. capreoli paired pyriforms resembled each other closely when in sika deer, but B. odocoilei pyriforms in white-tailed deer were slightly different. Babesia odocoilei in white-tailed deer also differed from B. odocoilei and B. capreoli in sika deer in the frequency of its developmental forms. Indirect immunofluorescence antibody test titres showed that there was some antigen cross-reactivity, but not as much as between B. capreoli and the bovine parasite, B. divergens. The Babesia spp. from deer that we studied appear to be distinct but related species. The low infectivity of B. odocoilei for a splenectomised sika deer suggests that sika deer in North America are probably not very susceptible to this parasite in the wild. 相似文献
70.